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Amplification and cloning of putative reverse transcriptase genes fromtricholoma spp. by polymerase chain reaction

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Mycoscience

Abstract

We previously identified a long terminal repeat retroelement from the ectomycorrhizal homobasidiomyceteTricholoma matsutake. In the present study the reverse transcriptase gene, a hallmark of retroelements, was amplified by polymerase chain reaction from various species of ectomycorrhizalTricholoma, and cloned. The results suggest that retroelements are shared in common in this fungal group rather than unique toT. matsutake.

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Authors and Affiliations

  1. Division of Bio-Resource Development, Forestry & Forest Products Research Institute, P.O. Box 16, 305-8687, Tsukuba-Norin, Japan

    Hitoshi Murata

  2. School of Agricultural Sciences, Shinshu University, 399-4598, Nagano, Minami Minowa 8304, Japan

    Akiyoshi Yamada

Authors
  1. Hitoshi Murata
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  2. Akiyoshi Yamada
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Correspondence to Hitoshi Murata.

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Murata, H., Yamada, A. Amplification and cloning of putative reverse transcriptase genes fromtricholoma spp. by polymerase chain reaction. Mycoscience 42, 395–398 (2001). https://doi.org/10.1007/BF02461223

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  • DOI: https://doi.org/10.1007/BF02461223

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