Abstract
Some properties of an extracellular lipase produced byLactobacillus delbrueckii subsp.bulgaricus were studied. Maximum enzyme activity was found against olive and butter oil as enzyme substrates. Addition of 9% acacia gum, 0.1% Na-deoxycholate and 0.01 M CaCl2 to the enzyme reaction mixture increased-lipase activity from 5.3 to 14.5 (FFA/mg protein/minute) at pH 6.0 and at 40° C. Maximum lipase production was reached in the presence of glucose as a sole source of carbon, wheat bran as nitrogen source, olive oil as a sole lipid source and butyric acid as fatty acid supporting the growth medium. An initial pH value of the culture medium of 6.0 and a temperature of 35° C gave the highest lipolytic activity.
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El-Sawah, M.M.A., Sherief, A.A. & Bayoumy, S.M. Enzymatic properties of lipase and characteristics production byLactobacillus delbrueckii subsp.bulgaricus . Antonie van Leeuwenhoek 67, 357–362 (1995). https://doi.org/10.1007/BF00872935
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DOI: https://doi.org/10.1007/BF00872935