Abstract
Lipases are esterases that occur widely in nature, yet those with commercial relevance are exclusively from microbial origin. Glycerol and long-chain fatty acids are the products after hydrolysis of esters bonds in saponifiable lipids catalyzed by lipases. In this work, we describe lipase/esterase activity contained in cell-free fractions from thermophilic bacteria, cultured in medium containing olive oil. Analysis of the cell-free fractions by electrotransference zymography, using tributyrin as substrate, revealed bands corresponding to lipase activity. The method is simple, fast, and inexpensive.
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Acknowledgments
This research was partially funded by the Consejo de Desarrollo Científico y Humanístico (CDCH-UAC-373-2010), University of Carabobo and by LOCTI. The authors thank the kind assistance of M.Sc. V. Storaci and D. Quintero.
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Tapizquent, M. et al. (2017). Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity. In: Wilkesman, J., Kurz, L. (eds) Zymography. Methods in Molecular Biology, vol 1626. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7111-4_28
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DOI: https://doi.org/10.1007/978-1-4939-7111-4_28
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