Abstract
Human neuronal cell cultures are essential tools for biological and preclinical studies of our nervous system. Since we have very limited access to primary human neural samples, derivation of proliferative neural progenitor cells (NPCs) from cells harvested by minimally invasive sampling is a key issue. Here we describe a “shortcut” method to establish proliferative NPC cultures directly from peripheral blood mononuclear cells (PBMCs) via interrupted reprogramming. In addition, we provide procedures to characterize the NPC stage.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bayart E, Cohen-Haguenauer O (2013) Technological overview of iPS induction from human adult somatic cells. Curr Gene Ther 13:73–92
Csobonyeiova M, Polak S, Zamborsky R, Danisovic L (2017) iPS cell technologies and their prospect for bone regeneration and disease modeling: a mini review. J Adv Res 8:321–327
Hong YJ, Do JT (2019) Neural lineage differentiation from pluripotent stem cells to mimic human brain tissues. Front Bioeng Biotechnol 7:400
Hussein SM et al (2014) Genome-wide characterization of the routes to pluripotency. Nature 516:198–206
Ebrahimi B (2016) Engineering cell fate: spotlight on cell-activation and signaling-directed lineage conversion. Tissue Cell 48:475–487
Zhu S, Wang H, Ding S (2015) Reprogramming fibroblasts toward cardiomyocytes, neural stem cells and hepatocytes by cell activation and signaling-directed lineage conversion. Nat Protoc 10:959–973
Drouin-Ouellet J, Pircs K, Barker RA, Jakobsson J, Parmar M (2017) Direct neuronal reprogramming for disease modeling studies using patient-derived neurons: what have we learned? Front Neurosci 11:530
Szabó E et al (2020) Functional comparison of blood-derived human neural progenitor cells. Int J Mol Sci 21:9118
Péntek A, Pászty K, Apáti Á (2016) Analysis of intracellular calcium signaling in human embryonic stem cells. Methods Mol Biol 1307:141–147
Acknowledgments
The authors thank Beáta Haraszti and Bálint Jezsó for technical assistance. This research was funded by the National Research, Development and Innovation Office [grant numbers: the National Brain Research Program (NAP) of Hungary—KTIA_NAP_13-1-2013-0001 to LH, 2017-1.2.1-NKP-2017-00002 to ÁA and LH; as well as Hungarian Scientific Research Fund (OTKA)—K128369 to ÁA, K128123 to LH, and PD128830 to ZsE].
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Szabó, E., Juhász, F., Homolya, L., Erdei, Z., Apáti, Á. (2021). Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_386
Download citation
DOI: https://doi.org/10.1007/7651_2021_386
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2118-9
Online ISBN: 978-1-0716-2119-6
eBook Packages: Springer Protocols