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Induced Pluripotent Stem (iPS) Cells

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Andras Nagy

    1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Kursad Turksen

    1. Ottawa, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2454)

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About this book

This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

Keywords

Table of contents (56 protocols)

  1. Front Matter

    Pages i-xxiii
    Download chapter PDF

  2. Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation

    • Davide Marotta, Chandrika Rao, Valentina Fossati
    Pages 1-15

  3. Generation of Induced Pluripotent Stem Cells from Human Bone Marrow–Derived Mesenchymal Stem Cells

    • Duygu Koyuncu Irmak, Erdal Karaoz
    Pages 17-29

  4. Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures

    • Bernat del Olmo, Daria Merkurjev, Likun Yao, Mel·lina Pinsach-Abuin, Ivan Garcia-Bassets, Angels Almenar-Queralt
    Pages 31-47

  5. Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

    • Varvara Chalmantzi, Chara Simitzi, Angelos Papadopoulos, Eleni Bagli, Carol Murphy, Emmanuel Stratakis et al.
    Pages 49-59

  6. Porcine iPSC Generation: Testing Different Protocols to a Successful Application

    • Oriol Iborra-Egea, Daina Martínez-Falguera, Santiago Roura, Antoni Bayes-Genis, Ángel Raya, Carolina Gálvez-Montón
    Pages 61-81

  7. Efficient High-Density hiPSCs Expansion in Simple Dialysis Device

    • Fuad Gandhi Torizal, Hyunjin Choi, Marie Shinohara, Yasuyuki Sakai
    Pages 83-94

  8. Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells

    • José González-Martínez, Marcos Malumbres
    Pages 95-107

  9. Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells

    • Hao Zhang, Mengcheng Shen, Joseph C. Wu
    Pages 109-115

  10. Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

    • Axel J. Chu, Chinten James Lim
    Pages 117-126

  11. 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells

    • Mariana A. Branco, Tiago P. Dias, João P. Cotovio, Carlos A. V. Rodrigues, Tiago G. Fernandes, Joaquim M. S. Cabral et al.
    Pages 127-143

  12. Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    • Sarkawt Hamad, Daniel Derichsweiler, Jürgen Hescheler, Kurt Pfannkuche
    Pages 145-161

  13. Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology

    • Qasim A. Majid, Barbara Orsolits, Lotta Pohjolainen, Zsófia Kovács, Gábor Földes, Virpi Talman
    Pages 163-196

  14. Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes

    • Sheeja Rajasingh, Vinoth Sigamani, Narasimman Gurusamy, Johnson Rajasingh
    Pages 197-212

  15. An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms

    • Mohammad Izadifar, Tünde Berecz, Ágota Apáti, Andras Nagy
    Pages 213-230

  16. Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells

    • Mégane Delourme, Natacha Broucqsault, Kilian Mazaleyrat, Frédérique Magdinier
    Pages 231-239

  17. Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming

    • Eszter Szabó, Flóra Juhász, László Homolya, Zsuzsa Erdei, Ágota Apáti
    Pages 241-255

  18. Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells

    • Manale Karam, Essam M. Abdelalim
    Pages 257-271

  19. The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts

    • Francesco Santarella, Fergal J. O’Brien, Jonathan A. Garlick, Cathal J. Kearney
    Pages 273-283

  20. 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells

    • Tomoko Miyake, Mikio Shimada
    Pages 285-295
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Editors and Affiliations

  • Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada

    Andras Nagy

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Induced Pluripotent Stem (iPS) Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Andras Nagy, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2119-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2118-9Published: 23 June 2022

  • Softcover ISBN: 978-1-0716-2121-9Published: 24 June 2023

  • eBook ISBN: 978-1-0716-2119-6Published: 22 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXIII, 835

  • Number of Illustrations: 37 b/w illustrations

  • Topics: Stem Cells, Developmental Biology

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