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Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells

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Human Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1307))

Abstract

Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can be used for other pluripotent cell types (such as iPSC) or their differentiated offspring as well.

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Acknowledgments

The authors appreciate the gift of HUES9 cell line by Dr. Douglas Melton, HHMI. This work was supported by the Hungarian Scientific Research Fund [grant numbers NK83533 and CK80283] and by the National Development Agency [grant numbers KTIA_AIK_12-1-2012-0025, KMR_12-1-2012-0112].

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Authors and Affiliations

  1. Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Diószegi 64, 1113, Budapest, Hungary

    Adrienn Péntek & Ágota Apáti

  2. Molecular Biophysics Research Group of the Hungarian Academy of Sciences and Department of Biophysics, Semmelweis University, Budapest, Hungary

    Katalin Pászty & Ágota Apáti

Authors
  1. Adrienn Péntek
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  2. Katalin Pászty
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  3. Ágota Apáti
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Corresponding author

Correspondence to Ágota Apáti .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2014 Springer Science+Business Media New York

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Péntek, A., Pászty, K., Apáti, Á. (2014). Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1307. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_68

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  • DOI: https://doi.org/10.1007/7651_2014_68

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2667-1

  • Online ISBN: 978-1-4939-2668-8

  • eBook Packages: Springer Protocols

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