Abstract
In 2006, 2007, and 2008, Alturas, Russet Burbank, FL1867, FL1879, Russet Norkotah, Ranger Russet, Shepody, and Umatilla Russet potatoes were planted at a research farm near Moxee, WA. Plants were allowed to become infested with native populations of the beet leafhopper which are known vectors of the Columbia Basin potato purple top phytoplasma, a member of phytoplasma group 16SrVI, in this region. Symptomatic plants were tested by PCR for the presence of the phytoplasma and positive plants were identified with flags. Tubers were harvested from positive plants and tested for the presence of phytoplasma. A subset of phytoplasma-positive tubers was planted in a greenhouse and the resulting daughter plants were tested for phytoplasma. The frequency of tuber transmission ranged from 4% to 96% among the eight cultivars over the 3 year period. Transmission of the phytoplasma from infected tubers to daughter plants ranged from 0% to 50% depending upon cultivar and year. In two of the 3 years, Russet Burbank showed the lowest rate of tuber transmission among the eight cultivars. Across the eight cultivars an average of 22%, 15%, and 16% of infected tubers gave rise to infected daughter plants in 2006, 2007, and 2008, respectively.
Resumen
Se plantaron en un campo experimental cerca de Moxee, WA, en 2006, 2007, y 2008, papas de las variedades Alturas, RussetBurbank, FL1867, FL 1879, RussetNorkotah, RangerRusset, Shepody y UmatillaRusset. Se permitió que las plantas se infestaran con poblaciones nativas de chicharritas del betabel, que se sabe son vectores del fitoplasma de la punta morada de la papa, miembro del grupo de fitoplasmas 16SrVI, en la región de la rivera del Columbia. A las plantas sintomáticas se les hizo la prueba de PCR para la presencia del fitoplasma, y a las positivas se les identificó con banderas. Se cosecharon los tubérculos de las plantas positivas y se probaron para la presencia del fitoplasma. Se plantó un subgrupo de tubérculos positivos en invernadero y las plantas resultantes se probaron para fitoplasmas. La frecuencia de transmisión por tubérculo tuvo una amplitud entre el 4 y el 96% entre las ocho variedades a lo largo del periodo de los tres años. La transmisión del fitoplasma de tubérculos infectados a plantas hijas varió de 0 a 50% dependiendo de la variedad y del año. En dos de los tres años, RussetBurbank mostró el nivel más bajo de transmisión por tubérculo entre las ocho variedades. Considerando todas las variedades, se obtuvo un promedio de 22%, 15% y 16% de tubérculos infectados que dieron origen a plantas hijas enfermas en 2006 a 2008, respectivamente.
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Partial funding for this project was provided by the Washington State Potato Commission and the USDA-ARS State Cooperative Potato Research Program. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer.
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Crosslin, J.M., Hamlin, L.L., Buchman, J.L. et al. Transmission of Potato Purple Top Phytoplasma to Potato Tubers and Daughter Plants. Am. J. Pot Res 88, 339–345 (2011). https://doi.org/10.1007/s12230-011-9199-y
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DOI: https://doi.org/10.1007/s12230-011-9199-y