Abstract
At times it may be desirable to determine the importance, rather than the actual values, of general (GCA) and specific combining ability (SCA) in parental materials. In screening large populations for disease resistance over years it may be difficult or impossible to accurately estimate these effects. Once a clone has been found to be susceptible it is usually discarded or lost to the disease, but multiple evaluations are desired before declaring a clone resistant. The purpose of this study was to determine the importance of GCA and SCA for late blight resistance in tetraploid potatoes. Five late blight resistant females were crossed to four males in a design II mating scheme to generate 20 families. Progeny were evaluated for foliar late blight from 2002 to 2004. In the first 2 years progeny that had ≥90% late blight infected foliage were discarded; progeny with <90% infected foliage were retested. Sixty-seven clones had no more than 50% infected foliage each year. Individual χ 2 tests demonstrated the significance of GCA in four of the five female parents, however, GCA was not significant in any of the male parents. The proportion of resistant progeny from the female and male parents was used to calculate the expected proportion of resistant progeny for each parental combination. The probbnml function in SAS was used to calculate the probability that the actual number of resistant clones observed was as great or greater than expected. SCA was important in four of the families. Although this method does not give estimates of GCA or SCA, it does determine if GCA or SCA are significant and which parents or parental combinations have significant GCA or SCA, respectively.
Resumen
A veces es deseable determinar la importancia, en lugar de los valores, de la habilidad de combinación general (GCA) y específica (SCA, por sus siglas en inglés) en los progenitores. Al tamizar poblaciones grandes para resistencia a la enfermedad año tras año puede ser difícil o imposible estimar estos efectos en forma precisa. Cuando un clon es hallado susceptible éste es generalmente descartado o perdido a la enfermedad, pero son deseables evaluaciones múltiples antes de declarar a un clon como resistente. El propósito de este estudio fue determinar la importancia de GCA y SCA para resistencia al tizón tardío en papas tetraploides. Cinco progenitores femeninos resistentes fueron cruzados con cuatro progenitores masculinos en un esquema de apareamiento diseño II para generar 20 familias. La progenie fue evaluada para tizón tardío foliar del 2002 al 2004. En los dos primeros años la progenie que tuvo ≥90% de follaje infectado por tizón tardío fue descartada; la progenie con <90% de follaje infectado fue reevaluado. Sesenta y siete clones tuvieron no más del 50% del follaje infectado cada año. La prueba individual χ 2 demostró la importancia del GCA en cuatro progenitores, sin embargo, el valor del GCA no fue significativo en ninguno de los progenitores masculinos. La proporción de progenie resistente de los progenitores femeninos y masculinos fue usada para calcular la proporción esperada de progenie resistente para cada combinación de progenitores. La función probbnml de SAS fue usada para calcular la probabilidad que el número de clones resistentes observado era tan grande o más que el esperado. La SCA fue importante en cuatro familias. Aunque este método no da estimados de GCA o SCA, determina si GCA o SCA son significativas y cuáles progenitores o combinaciones de progenitores tienen GCA o SCA significativas, respectivamente.
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Abbreviations
- GCA:
-
general combining ability
- SCA:
-
specific combining ability
- AUDPC:
-
area under the disease progress curve
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Acknowledgements
This research was funded in part by the USDA/ARS Potato Special Grants Program. Technical assistance was provided by Mike Peck, Sara May, Karl DeLong, Merle Bragg, Bonnie Adams, Karen Frazier, and Diane Fleck.
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Haynes, K.G., Christ, B.J. & Vinyard, B.T. Determining the Importance of Combining Ability for Late Blight Resistance in Early Generations of Potato Breeding When Susceptible Clones are Discarded. Am. J. Pot Res 85, 445–454 (2008). https://doi.org/10.1007/s12230-008-9051-1
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DOI: https://doi.org/10.1007/s12230-008-9051-1