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Signal Transduction — Single Cell Techniques

  • Book
  • © 1998

Overview

Editors:
  1. Bert Duijn

    1. Center for Phytotechnology RUL/TNO, Leiden, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

  2. Anneke Wiltink

    1. Department of Physiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (31 chapters)

  1. Front Matter

    Pages I-XXVII
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  2. Handling of Cells in Single Cell Experiments

    1. Front Matter

      Pages 1-1
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    2. Maintaining Cells Under the Microscope

      • Can Ince
      Pages 3-8

    3. Simple Perfusion Chambers for Single Cell Measurements

      • Kei Inouye
      Pages 9-18

    4. Temperature Control on the Stage of the Microscope

      • Arie de Vos, Bert Van Duijn, Anneke Wiltink
      Pages 19-23

    5. A Modified U-Tube Drug Application System

      • Misa Dzoljic
      Pages 24-30

    6. Laser Microsurgery as a Tool in Single Cell Research

      • Pettie P. Booij, Albertus H. De Boer
      Pages 31-40

  3. Ion Channel and Membrane Potential Measurements Using the Patch-Clamp Technique

    1. Front Matter

      Pages 41-41
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    4. II-3 Whole Cell Measurements

      1. Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations
        • Zheng Wang, Dirk L. Ypey, Rutgeris J. Van den Berg
        Pages 187-206
      2. Perforated Patch-Clamp Technique in Heart Cells
        • E. Etienne Verheijck
        Pages 207-213
      3. Measurement and Analysis of Different Aspects of Potassium Currents in Human Lymphocytes
        • Rezso Gáspár Jr., Zoltan Varga, György Panyi, Zoltan Krasznai, Carlo Pieri, Sandor Damjanovich
        Pages 214-235
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Keywords

About this book

A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

Editors and Affiliations

  • Center for Phytotechnology RUL/TNO, Leiden, The Netherlands

    Bert Duijn

  • Department of Physiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands

    Anneke Wiltink

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