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Shoot Organogenesis, Genetic Stability, and Secondary Metabolite Production of Micropropagated Digitalis purpurea

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Digitalis purpurea L. is a cardenolide-producing medicinal and ornamental plant. Cardenolides, like digoxin, are commonly used to treat congestive heart failure, cardiac arrhythmia, and atrial fibrillation. More recently, its cytotoxic activity on several types of cancer and antiviral effect have been confirmed. As chemical synthesis is not viable, D. purpurea plants are one of the major sources of cardenolides for the pharmaceutical industry. However, cardenolide content is highly variable under natural conditions. Therefore, D. purpurea in vitro culture has been a focus of research since the second half of the twentieth century. This chapter is a compendium of these reports with emphasis on the effects of morphogenesis, culture conditions, and in vitro culture medium composition on cardenolide content. Besides, some studies on the genetic stability of Digitalis plants are summarized. Finally, we describe the biotechnological approaches reported so far to obtain a higher yield of cardenolides in vitro, such as elicitation and metabolic engineering, both recognized as promising strategies.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

2iP:

N6-[2-Isopentenyl] adenine

ABA:

Abscisic acid

BA:

Benzyladenine

BTOA:

2-Benzothiazole-oxyacetic acid

DW:

Dry weight

FW:

Fresh weight

GA3:

Gibberellic acid

IAA:

Indoleacetic acid

LED:

Light-emitting diode

LS medium:

Linsmaier and Skoog medium

MS medium:

Murashige and Skoog medium

NAA:

Naphthaleneacetic acid

PEG:

Polyethylene glycol

RAPD:

Random amplified polymorphic DNA

RuBisCO:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

TDZ:

Thidiazuron

TIS:

Temporary immersion system

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Author information

Authors and Affiliations

  1. Departamento de Biología, Facultad de Ciencias Agropecuarias, Universidad Central Marta Abreu de Las Villas (UCLV), Santa Clara, Cuba

    Elizabeth Kairuz

  2. Instituto de Biotecnología de las Plantas (IBP), Universidad Central Marta Abreu de Las Villas, Santa Clara, Cuba

    Elizabeth Kairuz & Naivy Pérez-Alonso

  3. Laboratory of Plant Genetics, Vrije Universiteit Brussel (VUB), Brussels, Belgium

    Elizabeth Kairuz & Geert Angenon

  4. Florida Crystals Corp, Belle Glade, FL, USA

    Elio Jiménez

  5. Sociedad de Investigación y Servicios BioTECNOS Ltda, San Javier, Chile

    Borys Chong-Pérez

Authors
  1. Elizabeth Kairuz
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  2. Naivy Pérez-Alonso
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  3. Geert Angenon
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  4. Elio Jiménez
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  5. Borys Chong-Pérez
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Corresponding author

Correspondence to Borys Chong-Pérez .

Editor information

Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Kairuz, E., Pérez-Alonso, N., Angenon, G., Jiménez, E., Chong-Pérez, B. (2020). Shoot Organogenesis, Genetic Stability, and Secondary Metabolite Production of Micropropagated Digitalis purpurea. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_16-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_16-1

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  • Print ISBN: 978-3-030-11253-0

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