Abstract
Barley is an important crop both for brewing and for animal feed. In addition to conventional breeding, in vitro culture is a useful technology for the improvement of barley quality. For example, a number of methods for barley anther and microspore culture have been published for the production of doubled haploid lines for use in plant breeding and in genetic analysis (1).
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References
Pickering, A. R. and Devaux, P (1992) Haploid production: Approaches and use in plant breeding, in Barley Genetics, Biochemistry, Molecular Biology and Biotechnology (Shewry, P. R., ed.), CAB International, Wallingfold, UK, pp. 519–547.
Vasil, I. K. (1994) Molecular improvement of cereals. Plant Mol Biol. 25, 925–937
Krautwig, B. and Lörz, H (1995) Cereal protoplasts Plant Sci 111, 1–10.
Fromm, M. E., Morrish, F., Armstrong, C, Williams, R., Thomas, J., and Klein, T. M. (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8, 833–839
Shimamoto, K., Terada, R, Izawa, T., and Fujimoto, H. (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338, 274–276.
Yang, Z. Q., Shikanai, T., Mori, K., and Yamada, Y. (1989) Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.). Theor. Appl. Genet 77, 305–310.
Jähne, A., Lazzeri, P. A., and Lorz H. (1991) Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley Hordeum vulgare L.). Plant Cell Rep 10, 1–6.
Lorz, H. and Lazzeri, P. A (1992) In vitro regeneration and genetic transformation of barley. Barley Genet VI II, 807–815.
Lazzeri, P. A., Brettschneider, R., Lohrs, R, and Lorz, H. (1991) Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theor Appl. Genet. 81, 437–444.
Kihara, M. and Funatsuki, H. (1994) Fertile plant regeneration from barley (Hordeum vulgare L.) protoplasts isolated from long-term suspension culture. Breeding Sci. 44, 157–160.
Funatsuki, H. and Kihara, M. (1994) Influence of primary callus induction conditions on the establishment of barley cell suspensions yielding regenerable protoplasts. Plant Cell Rep 13, 551–555
Funatsuki, H., Kuroda, H., Kihara, M., Lazzeri, P. A., Muller, E., and Lorz, H (1995) Fertile transgenic barley generated by direct gene transfer to protoplasts. Theor. Appl. Genet. 91, 707–712.
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© 1999 Humana Press Inc., Totowa, NJ
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Kihara, M., Funatsuki, H., Ito, K., Lazzeri, P.A. (1999). Somatic Embryogenesis in Barley Suspension Cultures. In: Hall, R.D. (eds) Plant Cell Culture Protocols. Methods In Molecular Biology™, vol 111. Humana Press. https://doi.org/10.1385/1-59259-583-9:43
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DOI: https://doi.org/10.1385/1-59259-583-9:43
Publisher Name: Humana Press
Print ISBN: 978-0-89603-549-2
Online ISBN: 978-1-59259-583-9
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