Abstract
Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems. Here we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents.
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Acknowledgments
Bastiaan Lobbezoo and Steve Burton for valuable discussions.
ProMetic BioSciences Ltd is a trademark of ProMetic BioSciences Ltd. Mimetic Blue® is registered with the US Patent and Trademark Office. AlbuPure® and Albufuse® are registered trademarks of Novozymes Biopharma DK A/S. AlbuPure® is sold under license for the use in the purification of albumin fusion proteins with no warranty of merchantability or fitness for a particular purpose.
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Williams, S., Morton, P., Baines, D. (2014). Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins. In: Labrou, N. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 1129. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-977-2_16
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DOI: https://doi.org/10.1007/978-1-62703-977-2_16
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