Protein Downstream Processing

Design, Development and Application of High and Low-Resolution Methods

  • Nikolaos E. Labrou

Part of the Methods in Molecular Biology book series (MIMB, volume 1129)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Screening and Design Purification Strategies

    1. Front Matter
      Pages 1-1
    2. Nikolaos E. Labrou
      Pages 3-10
    3. Ignacio Amadeo, Laura Mauro, Eduardo Ortí, Guillermina Forno
      Pages 11-27
    4. Anurag S. Rathore, Rahul Bhambure
      Pages 29-37
    5. Anurag S. Rathore, Sampath Muthukumar
      Pages 39-44
    6. Charlotte Cabanne, Xavier Santarelli
      Pages 45-51
    7. Sunil Kumar, Narayan S. Punekar
      Pages 53-65
  3. Low-Resolution Protein Purification Methods

  4. High-Resolution Protein Purification Methods

    1. Front Matter
      Pages 145-145
    2. Ana Sofia Pina, Íris L. Batalha, Ana Cecília A. Roque
      Pages 147-168
    3. Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, Paola Loguercio Polosa
      Pages 169-180

About this book


Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.


chromatography electrophoretic techniques protein purification protein recovery recombinant enzymes

Editors and affiliations

  • Nikolaos E. Labrou
    • 1
  1. 1.Laboratory of Enzyme TechnologyAgricultural University of Athens Department of Agr. BiotechnologyAthensGreece

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-976-5
  • Online ISBN 978-1-62703-977-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book