Abstract
Cell shape and substrate rigidity play critical roles in regulating cell behaviors and fate. Controlling cell shape on elastic adhesive materials holds great promise for creating a physiologically relevant culture environment for basic and translational research and clinical applications. However, it has been technically challenging to create high-quality adhesive patterns on compliant substrates. We have developed an efficient and economical method to create precise micron-scaled adhesive patterns on the surface of a hydrogel (Rape et al., Biomaterials 32:2043–2051, 2011). This method will facilitate the research on traction force generation, cellular mechanotransduction, and tissue engineering, where precise controls of both materials rigidity and adhesive patterns are important.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tambe DT, Corey-Hardin C, Angelini TE et al (2011) Collective cell guidance by cooperative intercellular forces. Nat Mater 10:469–475
Janmey PA, Miller RT (2011) Mechanisms of mechanical signaling in development and disease. J Cell Sci 124:9–18
Chen CS, Mrksich M, Huang S et al (1997) Geometric control of cell life and death. Science 276:1425–1428
Singhvi R, Kumar A, Lopez G et al (1994) Engineering cell shape and function. Science 264:696–698
Dike L, Chen C, Mrksich M et al (1999) Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35: 441–448
McBeath R, Pirone DM, Nelson CM et al (2004) Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment. Dev Cell 6:483–495
Kilian KA, Bugarija B, Lahn BT et al (2010) Geometric cues for directing the differentiation of mesenchymal stem cells. Proc Natl Acad Sci 107:4872–4877
Wang H-B, Dembo M, Wang Y-L (2000) Substrate flexibility regulates growth and apoptosis of normal but not transformed cells. Am J Physiol Cell Physiol 279:C1345–C1350
Engler AJ, Sen S, Sweeney HL et al (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689
Pelham RJ, Wang Y-l (1997) Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci 94:13661–13665
Rape A, Guo W-H, Wang Y-L (2011) Responses of cells to adhesion-mediated signals: a universal mechanism. In: Wagoner JA, Harley BAC (eds) Mechanobiology of cell-cell and cell-matrix interactions. Springer, USA, pp 1–10
Wang Y-L, Pelham RJ Jr (1998) Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells. In: Richard BV (ed) Methods in enzymology. Academic, New York, pp 489–496
Damljanovic V, Lagerholm BC, Jacobson K (2005) Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. Biotechniques 39:847–851
Guo W-h, Wang Y-I (2007) Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals. Mol Biol Cell 18:4519–4527
Wang N, Ostuni E, Whitesides GM et al (2002) Micropatterning tractional forces in living cells. Cell Motil Cytoskeleton 52:97–106
Vignaud T, Galland R, Tseng Q et al (2012) Reprogramming cell shape with laser nano-patterning. J Cell Sci 125:2134–2140
Rape AD, Guo W-h, Wang Y-l (2011) The regulation of traction force in relation to cell shape and focal adhesions. Biomaterials 32:2043–2051
Fischer B, Thomas KB, Dorner F (1998) Collagen covalently immobilized onto plastic surfaces simplifies measurement of von Willebrand factor-collagen binding activity. Ann Hematol 76:159–166
Li F, Li B, Wang Q-M et al (2008) Cell shape regulates collagen type I expression in human tendon fibroblasts. Cell Motil Cytoskeleton 65:332–341
Reinhart-King CA, Dembo M, Hammer DA (2003) Endothelial cell traction forces on RGD-derivatized polyacrylamide substrata. Langmuir 19:1573–1579
Guo W-H, Wang Y-l (2011) Micropatterning cell-substrate adhesions using linear polyacrylamide as the blocking agent. Cold Spring Harb Protoc 2011(3):prot5582
Acknowledgement
This work was supported by grant GM-32476 from the National Institutes of Health to Y.L.W.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Zhang, J., Guo, Wh., Rape, A., Wang, Yl. (2013). Micropatterning Cell Adhesion on Polyacrylamide Hydrogels. In: Baudino, T. (eds) Cell-Cell Interactions. Methods in Molecular Biology, vol 1066. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-604-7_13
Download citation
DOI: https://doi.org/10.1007/978-1-62703-604-7_13
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-603-0
Online ISBN: 978-1-62703-604-7
eBook Packages: Springer Protocols