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Cell-Cell Interactions

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Troy A. Baudino

    1. Texas A&M Health Science Center, Temple, USA

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  • Present current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1066)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Proteomics Analysis of Contact-Initiated Eph Receptor–Ephrin Signaling

    • Claus Jorgensen, Alexei Poliakov
    Pages 1-16

  3. Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes

    • George E. Davis, Dae Joong Kim, Chun-Xia Meng, Pieter R. Norden, Katherine R. Speichinger, Matthew T. Davis et al.
    Pages 17-28

  4. Analyzing Cell–Cell Interactions in 3-Dimensional Adhesion Assays

    • Stephanie L. K. Bowers, Troy A. Baudino
    Pages 29-43

  5. Production of Spontaneously Beating Neonatal Rat Heart Tissue for Calcium and Contractile Studies

    • Fnu Gerilechaogetu, Hao Feng, Honey B. Golden, Damir Nizamutdinov, Donald M. Foster, Shannon Glaser et al.
    Pages 45-56

  6. Paracrine Communication Between Mechanically Stretched Myocytes and Fibroblasts

    • Hao Feng, Fnu Gerilechaogetu, Honey B. Golden, Damir Nizamutdinov, Donald M. Foster, Shannon Glaser et al.
    Pages 57-66

  7. Assessing Blood–Brain Barrier Function Using In Vitro Assays

    • Joseph Bressler, Katherine Clark, Cliona O’Driscoll
    Pages 67-79

  8. Methods to Assess Tissue Permeability

    • Juan C. Ibla, Joseph Khoury
    Pages 81-88

  9. In Vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature

    • F. Bartsch, M. L. Kang, S. T. Mees, J. Haier, P. Gassmann
    Pages 89-101

  10. Cell Membrane Vesicles as a Tool for the Study of Direct Epithelial–Stromal Interaction: Lessons from CD147

    • Eric Gabison, Farah Khayati, Samia Mourah, Suzanne Menashi
    Pages 103-111

  11. Microencapsulation of Stem Cells to Study Cellular Interactions

    • Keith Moore, Adam Vandergriff, Jay D. Potts
    Pages 113-120

  12. Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies

    • Timothy N. Feinstein
    Pages 121-129

  13. Single Cell Analysis of Lipid Rafts

    • William T. Lee
    Pages 131-145

  14. Micropatterning Cell Adhesion on Polyacrylamide Hydrogels

    • Jian Zhang, Wei-hui Guo, Andrew Rape, Yu-li Wang
    Pages 147-156

  15. Measuring Cell–Cell Tugging Forces Using Bowtie-Patterned mPADs (Microarray Post Detectors)

    • Daniel M. Cohen, Mike T. Yang, Christopher S. Chen
    Pages 157-168

  16. Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells

    • Katharina Austen, Carleen Kluger, Andrea Freikamp, Anna Chrostek-Grashoff, Carsten Grashoff
    Pages 169-184

  17. Proteomic Analysis of the Left Ventricle Post-myocardial Infarction to Identify In Vivo Candidate Matrix Metalloproteinase Substrates

    • Andriy Yabluchanskiy, Yaojun Li, Lisandra E. de Castro Brás, Kevin Hakala, Susan T. Weintraub, Merry L. Lindsey
    Pages 185-199

  18. Back Matter

    Pages 201-204
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Keywords

About this book

In the second edition of Cell-Cell Interactions: Methods and Protocols, expert researchers in the field detail a collection of protocols to examine interactions between cells. These include protocols that focus on both in vivo and in vitro methods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and Practical, Cell-Cell Interactions: Methods and Protocol, Second Edition is a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for those that are new to the area.

Editors and Affiliations

  • Texas A&M Health Science Center, Temple, USA

    Troy A. Baudino

Bibliographic Information

  • Book Title: Cell-Cell Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Troy A. Baudino

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-604-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-603-0Published: 17 August 2013

  • Softcover ISBN: 978-1-4939-6049-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-604-7Published: 17 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 204

  • Number of Illustrations: 19 b/w illustrations, 21 illustrations in colour

  • Topics: Cell Biology, Cell Physiology

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