Abstract
Homeostatic maintenance of physiological and biochemical processes is a key requirement for survival and adaptive responses of multicellular organisms such as plants. These important processes are in part mediated by various plant enzymes and hormones, many of which are in part, controlled by cyclic nucleotides and/or other signalling molecules. Infrared gas analysis (IRGA) technique is one of the modern methods which allows for rapid and accurate measurements of cyclic nucleotide mediated photosynthetic responses to plant hormones, and thus makes it a powerful and useful tool to study aspects of downstream cell signalling events in plants. In this chapter the basic protocols enabling the use of the IRGA technique to study signalling molecules, such as cyclic nucleotides on photosynthetic responses, are outlined.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Johri MM (2008) Hormonal regulation in green plant lineage families. Physiol Mol Biol Plants 14:23–38
Vogler H, Kuhlemeier C (2003) Simple hormones but complex signalling. Curr Opin Plant Biol 6:51–56
Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 55:373–399
Sanders D, Pelloux J, Brownlee C, Harper JF (2002) Calcium at the crossroads of signaling. Plant Cell 14(Suppl):S401–S417
Newton RP, Smith CJ (2004) Cyclic nucleotides. Phytochemistry 65:2423–2437
Felle HH (2001) pH: signal and messenger in plant cells. Plant Biol 3:577–591
Besson-Bard A, Pugin A, Wendehenne D (2008) New insights into nitric oxide signaling in plants. Ann Rev Plant Biol 59:21–39
Meijer HJG, Munnik T (2003) Phospholipid-based signaling in plants. Ann Rev Plant Biol 54:265–306
Gehring C, Irving H (2003) Natriuretic peptides—a class of heterologous molecules in plants. Int J Biochem Cell Biol 35:1318–1322
Billington T, Pharmawati M, Gehring CA (1997) Isolation and immunoaffinity purification of biologically active plant natriuretic peptide. Biochem Biophys Res Commun 235:722–725
Maryani MM, Bradley G, Cahill D, Gehring C (2001) Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts. Plant Sci 161:443–452
Rafudeen S, Gxaba G, Makgoke G, Bradley G, Pironcheva G et al (2003) A role for plant natriuretic peptide immuno-analogues in NaCl- and drought-stress responses. Physiol Plant 119:554–562
Morse M, Pironcheva G, Gehring C (2004) AtPNP-A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation. FEBS Lett 556:99–103
Pharmawati M, Maryani MM, Nikolakopoulos T, Gehring CA, Irving HR (2001) Cyclic GMP modulates stomatal opening induced by natriuretic peptides and immunoreactive analogues. Plant Physiol Biochem 39:385–394
Ludidi N, Morse M, Sayed M, Wherrett T, Shabala S, Gehring C (2004) A recombinant plant natriuretic peptide causes rapid and spatially differentiated K+, Na+ and H+ flux changes in Arabidopsis thaliana roots. Plant Cell Physiol 45:1093–1098
Gottig N, Garavaglia BS, Daurelio LD, Valentine A, Gehring C, Orellano EG et al (2008) Xanthomonas axonopodis pv. citri uses a plant natriuretic peptide-like protein to modify host homeostasis. Proc Natl Acad Sci U S A 105:18631–18636
Ruzvidzo O, Donaldson L, Valentine A, Gehring C (2011) The Arabidopsis thaliana natriuretic peptide AtPNP-A is a systemic regulator of leaf dark respiration and signals via the phloem. J Plant Physiol 168:1710–1714
Wang YH, Gehring C, Cahill DM, Irving HR (2007) Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation. Funct Plant Biol 34:645–653
Hunt S (2003) Measurements of photosynthesis and respiration in plants. Physiol Plant 117:314–325
Caemmerer S, Farquhar GD (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153:376–387
Farquhar GD, Sharkey TD (1982) Stomatal conductance and photosynthesis. Ann Rev Plant Physiol 33:317–345
Acknowledgment
This material is based upon work supported financially by the National Research Foundation, South Africa.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Valentine, A., Ruzvidzo, O., Kleinert, A., Kang, Y., Bennedito, V. (2013). Infrared Gas Analysis Technique for the Study of the Regulation of Photosynthetic Responses. In: Gehring, C. (eds) Cyclic Nucleotide Signaling in Plants. Methods in Molecular Biology, vol 1016. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-441-8_19
Download citation
DOI: https://doi.org/10.1007/978-1-62703-441-8_19
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-440-1
Online ISBN: 978-1-62703-441-8
eBook Packages: Springer Protocols