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  • © 2013

Cyclic Nucleotide Signaling in Plants

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1016)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. Molecular Methods for the Study of Signal Transduction in Plants

    • Helen R. Irving, Chris Gehring
    Pages 1-11
  3. Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

    • Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi
    Pages 13-25
  4. Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

    • Heike Burhenne, Volkhard Kaever
    Pages 27-37
  5. In Vivo Imaging of cGMP in Plants

    • Jean-Charles Isner, Frans J. M. Maathuis
    Pages 57-65
  6. Calcium Imaging of the Cyclic Nucleotide Response

    • Martin R. McAinsh, Stephen K. Roberts, Lyudmila V. Dubovskaya
    Pages 107-119
  7. Identification and Quantitation of Signal Molecule-Dependent Protein Phosphorylation

    • Arnoud Groen, Ludivine Thomas, Kathryn Lilley, Claudius Marondedze
    Pages 121-137
  8. Comparative Gel-Based Phosphoproteomics in Response to Signaling Molecules

    • Claudius Marondedze, Kathryn Lilley, Ludivine Thomas
    Pages 139-154
  9. Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity

    • Victor Muleya, Janet I. Wheeler, Helen R. Irving
    Pages 175-194
  10. Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions

    • Alice K. Zelman, Adam Dawe, Gerald A. Berkowitz
    Pages 207-224
  11. Infrared Gas Analysis Technique for the Study of the Regulation of Photosynthetic Responses

    • Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang, Vagner Bennedito
    Pages 261-269

About this book

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.

Editors and Affiliations

  • , Division of Chemical and Life Sciences a, 4700 King Abdullah University of Science, Thuwal, Saudi Arabia

    Chris Gehring

Bibliographic Information

  • Book Title: Cyclic Nucleotide Signaling in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Chris Gehring

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-441-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-440-1Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-6291-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-441-8Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 273

  • Number of Illustrations: 16 b/w illustrations, 25 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access