Abstract
An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.
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Acknowledgments
We thank Kazuhito Akama, Toshiro Ito, and Tatsuo Kakimoto for valuable information about tissue culture media formulations, Cory Tobin and Paul Tarr for information and technical suggestions about FM dye staining, and Carolyn Ohno for information about seeds sterilization. This work was supported by the US National Science Foundation (Grant IOS-0846192 to E.M.M.) and the Japan Society for the Promotion of Science (to K.S.).
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Sugimoto, K., Meyerowitz, E.M. (2013). Regeneration in Arabidopsis Tissue Culture. In: De Smet, I. (eds) Plant Organogenesis. Methods in Molecular Biology, vol 959. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-221-6_18
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DOI: https://doi.org/10.1007/978-1-62703-221-6_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-220-9
Online ISBN: 978-1-62703-221-6
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