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High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants

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Abstract

A highly efficient and fast Agrobacterium-mediated leaf disc transformation system for the Arabidopsis thaliana L. genotype C24 was developed. This protocol is also amenable to other ecotypes - as could be shown for Landsberg erecta and Wassllewskija. Besides the hygromycin selection also the G418 and kanamycin selection were established. Furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as explant source for transformation experiments.

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Abbreviations

BAP:

6-Benzylaminopurine

CIM:

Callus induction medium

2.4D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

2-IP:

N6-γ,γ-Dimethylallyladenosine

HPT:

Hygromycin phosphotransferase

NAA:

α-Naphthaleneacetic acid

NPT II:

Neomycin phosphotransferase type II

SEM:

Shoot elongation medium

SIM:

Shoot induction medium

RIM:

Root induction medium

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Authors and Affiliations

  1. Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany

    Renate Schmidt & Lothar Willmitzer

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  1. Renate Schmidt
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  2. Lothar Willmitzer
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Communicated by H. Lörz

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Schmidt, R., Willmitzer, L. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants. Plant Cell Reports 7, 583–586 (1988). https://doi.org/10.1007/BF00272763

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  • DOI: https://doi.org/10.1007/BF00272763

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