Abstract
Currently, the most efficient detection and precise quantification of phytoplasmas is by real-time PCR. Compared to nested PCR, this method is less sensitive to contamination and is less work intensive. Therefore, a universal real-time PCR method will be valuable in screening programs and in other setups in which large numbers of samples are tested.
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References
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Christensen, N.M., Nyskjold, H., Nicolaisen, M. (2013). Real-Time PCR for Universal Phytoplasma Detection and Quantification. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_21
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DOI: https://doi.org/10.1007/978-1-62703-089-2_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-088-5
Online ISBN: 978-1-62703-089-2
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