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Real-Time PCR for Universal Phytoplasma Detection and Quantification

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Phytoplasma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 938))

Abstract

Currently, the most efficient detection and precise quantification of phytoplasmas is by real-time PCR. Compared to nested PCR, this method is less sensitive to contamination and is less work intensive. Therefore, a universal real-time PCR method will be valuable in screening programs and in other setups in which large numbers of samples are tested.

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Correspondence to Mogens Nicolaisen .

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Christensen, N.M., Nyskjold, H., Nicolaisen, M. (2013). Real-Time PCR for Universal Phytoplasma Detection and Quantification. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_21

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  • DOI: https://doi.org/10.1007/978-1-62703-089-2_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-088-5

  • Online ISBN: 978-1-62703-089-2

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