Methods and Protocols

  • Matt Dickinson
  • Jennifer Hodgetts

Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Matt Dickinson, Melanie Tuffen, Jennifer Hodgetts
    Pages 1-14
  3. Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, Nicoletta Contaldo
    Pages 33-39
  4. Jaroslava Přibylová, Josef Špak
    Pages 41-46
  5. Heather Kingdom
    Pages 47-59
  6. Phyllis Weintraub, Jürgen Gross
    Pages 61-72
  7. Domenico Bosco, Rosemarie Tedeschi
    Pages 73-85
  8. Sabrina Bertin, Domenico Bosco
    Pages 87-108
  9. Nancy M. Andrade, Nolberto L. Arismendi
    Pages 115-121
  10. Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, Marina Dermastia
    Pages 139-145
  11. Nigel A. Harrison, Robert E. Davis, Ericka E. Helmick
    Pages 147-158
  12. Bojan Duduk, Samanta Paltrinieri, Ing-Ming Lee, Assunta Bertaccini
    Pages 159-171
  13. Marta Martini, Ing-Ming Lee
    Pages 173-188
  14. Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová et al.
    Pages 189-204
  15. Matt Dickinson, Jennifer Hodgetts
    Pages 205-215
  16. Mogens Nicolaisen, Henriette Nyskjold, Assunta Bertaccini
    Pages 223-232

About this book


Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.


phytoplasma diseases phytoplasma genomes plant DNA plant pathology taxonomic groups

Editors and affiliations

  • Matt Dickinson
    • 1
  • Jennifer Hodgetts
    • 2
  1. 1.School of BiosciencesThe University of NottinghamNottinghamUnited Kingdom
  2. 2.The Food and Environment Research AgencySand HuttonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-088-5
  • Online ISBN 978-1-62703-089-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book