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DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

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Phytoplasma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 938))

Abstract

The 4′,6-diamidino-2-phenylindole (DAPI) stain technique is a simple method that was developed for confirming the presence of phytoplasmas in hand-cut or freezing microtome sections of infected tissues. DAPI binds AT-rich DNA preferentially, so that phytoplasmas, localized among phloem cells, can be visualized in a fluorescence microscope. The procedure is quick, easy to use, inexpensive, and can be used as a preliminary or quantitative method to detect or quantify phytoplasma-like bodies in infected plants.

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Author information

Authors and Affiliations

  1. Institute of Production and Plant Protection, Universidad Austral de Chile, Valdivia, Chile

    Nancy M. Andrade & Nolberto L. Arismendi

Authors
  1. Nancy M. Andrade
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  2. Nolberto L. Arismendi
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Corresponding author

Correspondence to Nancy M. Andrade .

Editor information

Editors and Affiliations

  1. School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Nottingham, LE12 5RD, United Kingdom

    Matt Dickinson

  2. The Food and Environment Research Agency, Sand Hutton, YO41 1LZ, United Kingdom

    Jennifer Hodgetts

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Andrade, N.M., Arismendi, N.L. (2013). DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_10

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  • DOI: https://doi.org/10.1007/978-1-62703-089-2_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-088-5

  • Online ISBN: 978-1-62703-089-2

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