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A new chromosome fluorescence banding technique combining DAPI staining with image analysis in plants

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Abstract

In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4′,6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this technique in the tested species including Hordeum vulgare L., Oryza officinalis, Wall & Watt, Triticum aestivum L., Lilium brownii, Brown, and Vicia faba L. During mitotic metaphase, the numbers of bands for the haploid genomes of these species were about 185, 141, 309, 456 and 194, respectively. Reproducibility analysis demonstrated that banding patterns within a species were stable at the same mitotic stage and they could be used for identifying specific chromosomes and chromosome regions. The band number fluctuated: the earlier the mitotic stage, the greater the number of bands. The technique enables genes to be mapped onto specific band regions of the chromosomes by only one fluorescence in situ hybridisation (FISH) step with no chemical banding treatments. In this study, the 45S and 5S rDNAs of some tested species were located on specific band regions of specific chromosomes and they were all positioned at the interbands with the new technique. Because no chemical banding treatment was used, the banding patterns displayed by the technique should reflect the natural conformational features of chromatin. Thus it could be expected that this technique should be suitable for all eukaryotes and would have widespread utility in chromosomal structure analysis and physical mapping of genes.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 39870423) and China Postdoctoral Science Foundation (no. 2003034496).

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Authors and Affiliations

  1. The Key Laboratory of the Ministry of Education of Plant Developmental Biology, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jing Yu Liu, Chao Wen She, Zhong Li Hu, Zhi Yong Xiong, Li Hua Liu & Yun Chun Song

  2. College of Life Sciences, Henan University, Kaifeng, 475001, People’s Republic of China

    Jing Yu Liu

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  1. Jing Yu Liu
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  2. Chao Wen She
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  3. Zhong Li Hu
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  4. Zhi Yong Xiong
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  5. Li Hua Liu
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Correspondence to Yun Chun Song.

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Communicated by P. Shaw

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Liu, J.Y., She, C.W., Hu, Z.L. et al. A new chromosome fluorescence banding technique combining DAPI staining with image analysis in plants. Chromosoma 113, 16–21 (2004). https://doi.org/10.1007/s00412-004-0289-1

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  • DOI: https://doi.org/10.1007/s00412-004-0289-1

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