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Chromatin Immunoprecipitation Assay as a Tool for Analyzing Transcription Factor Activity

  • Padmaja Gade
  • Dhan V. KalvakolanuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 809)

Abstract

Differential gene expression is facilitated by transcriptional regulatory mechanisms and chromatin modifications through DNA–protein interactions. One of the widely used assays to study this is chromatin immunoprecipitation (ChIP) assay, which enables analysis of association of regulatory molecules to specific promoters and histone modifications in vivo. This is of immense value as ChIP assays can provide glimpse of the regulatory mechanisms involved in gene expression in vivo. This article outlines the general strategies and protocols to study ChIP assays in differential recruitment of transcriptional factors (TFs) and also global analysis of transcription factor recruitment is discussed. Further, the applications of ChIP assays for discovering novel genes that are dependent on specific transcription factors were addressed.

Key words

ChIP dapk1 IFN-γ C/EBP-β Transcription factor activity Regulation of gene expression DNA–protein interactions Transcription factor recruitment 

Notes

Acknowledgments

This work is supported by NIH grants CA78282 and CA105005 to D.V.K.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Microbiology & Immunology, Greenebaum Cancer CenterUniversity of Maryland School of MedicineBaltimoreUSA

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