Transcriptional Regulation

Methods and Protocols

  • Ales Vancura

Part of the Methods in Molecular Biology book series (MIMB, volume 809)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Promoter Elements, Transcription Factors, and Pre-initiation Complex Assembly

    1. Front Matter
      Pages 1-1
    2. Ashish Juvekar, Sitharam Ramaswami, Subrata Manna, Tzu-Pei Chang, Adeel Zubair, Ivana Vancurova
      Pages 49-62
    3. Bing Tian, Jun Yang, Allan R. Brasier
      Pages 105-120
    4. Sitharam Ramaswami, Subrata Manna, Ashish Juvekar, Steven Kennedy, Ales Vancura, Ivana Vancurova
      Pages 121-134
    5. Chris Murgatroyd, Anke Hoffmann, Dietmar Spengler
      Pages 135-148
    6. Charles E. Massie, Ian G. Mills
      Pages 157-173
  3. Chromatin Structure

    1. Front Matter
      Pages 215-215
    2. Ryoko Harada, Alain Nepveu
      Pages 237-253
    3. Luciano Galdieri, John Moon, Ales Vancura
      Pages 255-265

About this book


Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world.  Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.


Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time.


ChIP assays Chromatin immunoprecipitation Chromatin structure Drosophila cells Gene expression Histone modifications Mass spectrometry Pre-initiation complex (PIC) Promoter elements RNA synthesis Transcription factors Yeast and mammalian cells

Editors and affiliations

  • Ales Vancura
    • 1
  1. 1., Department of Biological SciencesSt. John's UniversityQueensUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-375-2
  • Online ISBN 978-1-61779-376-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book