Abstract
The use of polyclonal antibodies enables the detection of proteins on a cellular and even subcellular level. Immunolocalization can be used on all pathosystems even if one or both partners of the interaction are unamenable to molecular tools like transformation. This chapter provides detailed information about how to obtain high quality antibodies, how to prepare samples, and finally how to detect the proteins. Methods for light and electron microscopy are presented.
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Acknowledgments
We are grateful to Heinz Vahlenkamp for technical assistance. This work was supported by various grants provided by the Deutsche Forschungsgemeinschaft.
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Kemen, E., Mendgen, K., Voegele, R.T. (2011). Immunolocalization of Pathogen Effectors. In: McDowell, J. (eds) Plant Immunity. Methods in Molecular Biology, vol 712. Humana Press. https://doi.org/10.1007/978-1-61737-998-7_17
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DOI: https://doi.org/10.1007/978-1-61737-998-7_17
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