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Plant Immunity

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. John M. McDowell

    1. , Plant Pathology, Physiol., & Weed Sci., Virginia Tech, Blacksburg, USA

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  • Emphasizes emerging technologies in plant immunity
  • Easily applied to a wide variety of plant-microbe interactions
  • Contains expert tips on troubleshooting and key implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 712)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

    • Patrick Cournoyer, S. P. Dinesh-Kumar
    Pages 1-8

  3. Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function

    • Peter Moffett
    Pages 9-20

  4. Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

    • Yiping Qi, Fumiaki Katagiri
    Pages 21-30

  5. Biochemical Purification of Native Immune Protein Complexes

    • James M. Elmore, Gitta Coaker
    Pages 31-44

  6. Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity

    • Mario Roccaro, Imre E. Somssich
    Pages 45-58

  7. Dose–Response to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in Arabidopsis

    • Xueqing Geng, David Mackey
    Pages 59-68

  8. Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis

    • Xue-Cheng Zhang, Walter Gassmann
    Pages 69-77

  9. Constructing Haustorium-Specific cDNA Libraries from Rust Fungi

    • Ann-Maree Catanzariti, Rohit Mago, Jeff Ellis, Peter Dodds
    Pages 79-87

  10. Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes

    • Richard S. Hussey, Guozhong Huang, Rex Allen
    Pages 89-107

  11. Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains

    • Brian H. Kvitko, Alan Collmer
    Pages 109-128

  12. A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores

    • Edgar Huitema, Matthew Smoker, Sophien Kamoun
    Pages 129-135

  13. Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis

    • John M. McDowell, Troy Hoff, Ryan G. Anderson, Daniel Deegan
    Pages 137-151

  14. Assaying Effector Function in Planta Using Double-Barreled Particle Bombardment

    • Shiv D. Kale, Brett M. Tyler
    Pages 153-172

  15. Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast

    • Yuanchao Wang, Qian Huang
    Pages 173-180

  16. Purification of Effector–Target Protein Complexes via Transient Expression in Nicotiana benthamiana

    • Joe Win, Sophien Kamoun, Alexandra M. E. Jones
    Pages 181-194

  17. Imaging Fluorescently Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue

    • Petra C. Boevink, Paul R. J. Birch, Stephen C. Whisson
    Pages 195-209

  18. Immunolocalization of Pathogen Effectors

    • Eric Kemen, Kurt Mendgen, Ralf T. Voegele
    Pages 211-225

  19. Laser Capture Microdissection of Nematode Feeding Cells

    • Nagabhushana Ithal, Melissa G. Mitchum
    Pages 227-240

  20. Laser Microdissection of Plant–Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling

    • Divya Chandran, Noriko Inada, Mary C. Wildermuth
    Pages 241-262
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Keywords

About this book

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes.  In  Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens.  These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.  Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

“This volume … describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. … Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field.” (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)

Editors and Affiliations

  • , Plant Pathology, Physiol., & Weed Sci., Virginia Tech, Blacksburg, USA

    John M. McDowell

Bibliographic Information

  • Book Title: Plant Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: John M. McDowell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61737-998-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61737-997-0Published: 02 March 2011

  • Softcover ISBN: 978-1-4939-5755-2Published: 23 August 2016

  • eBook ISBN: 978-1-61737-998-7Published: 26 February 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 295

  • Topics: Plant Sciences, Plant Pathology

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