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Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography

  • Protocol
Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 147))

Abstract

Immunoaffinity chromatography (IAC) has long been regarded as a highly specific method for the purification of biological agents. In this technique, a chromatographic column is used that contains antibodies or antibody-related reagents as the stationary phase. The high selectivity of antibodies in their interactions with other molecules, and the ability to produce antibodies against a wide range of solutes, has made IAC popular as a tool for the purification of biomolecules like hormones, peptides, enzymes, recombinant proteins, receptors, viruses, and subcellular components (1–8). In recent years, the high selectivity of IAC has also made it appealing as a means for developing a variety of specific analytical methods (3,9,10). An important item to consider in the development of any IAC method is the technique used for coupling the antibodies to the chromatographic support material. One common approach

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Authors and Affiliations

  1. Department of Chemistry, University of Nebraska, Lincoln, NE

    David S. Hage

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  1. David S. Hage
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Editors and Affiliations

  1. Department of Biopharmaceuticals, Hoffmann-La Roche Inc., Nutley, NJ

    Pascal Bailon , George K. Ehrlich , Wen-Jian Fung  & Wolfgang Berthold , ,  & 

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© 2000 Humana Press Inc.

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Hage, D.S. (2000). Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography. In: Bailon, P., Ehrlich, G.K., Fung, WJ., Berthold, W. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 147. Humana Press. https://doi.org/10.1007/978-1-60327-261-2_7

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  • DOI: https://doi.org/10.1007/978-1-60327-261-2_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-694-9

  • Online ISBN: 978-1-60327-261-2

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