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Single step oxidative binding of antibodies to hydrazide-modified eupergit C

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Abstract

A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix.

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Abbreviations

ADH:

adipic dihydrazide

CPA:

carboxypeptidase A

HRP:

horseradish peroxidase

mAb:

monoclonal antibody

PBS:

phosphate buffered saline, pH 7.4

TNBS:

Trinitrobenzene sulfonic acid.

References

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Life Sciences, Tel-Auiu university, Tel-Aviv, Israel

    Gideon Fleminger, Beka Solomon, Tamar Wolf & Eran Hadas

Authors
  1. Gideon Fleminger
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  2. Beka Solomon
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  3. Tamar Wolf
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  4. Eran Hadas
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Fleminger, G., Solomon, B., Wolf, T. et al. Single step oxidative binding of antibodies to hydrazide-modified eupergit C. Appl Biochem Biotechnol 26, 231–238 (1990). https://doi.org/10.1007/BF02921504

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  • DOI: https://doi.org/10.1007/BF02921504

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