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Affinity Chromatography

Methods and Protocols

  • Pascal Bailon
  • George K. Ehrlich
  • Wen-Jian Fung
  • Wolfgang Berthold

Part of the Methods in Molecular Biology book series (MIMB, volume 147)

Table of contents

  1. Front Matter
    Pages i-x
  2. Meir Wilchek, Irwin Chaiken
    Pages 1-6
  3. Magnus Strandh, HÅkan S. Andersson, Sten Ohlson
    Pages 7-23
  4. Cheryl L. Spence, Pascal Bailon
    Pages 25-39
  5. Alexander Schwarz
    Pages 49-56
  6. Gjalt W. Welling, Sytske Welling-Wester
    Pages 83-88
  7. Joseph Thalhamer, Peter Hammerl, Arnulf Hartl
    Pages 89-93
  8. Anuradha Subramanian
    Pages 95-104
  9. Göte Johansson
    Pages 105-117
  10. Xiao-Chuan Liu, William H. Scouten
    Pages 119-128
  11. Priya Sethu Chockalingam, Luis A. Jurado, F. Darlene, Harry W. Jarrett
    Pages 141-153
  12. Satoshi Minobe, Takeji Shibatani, Tetsuya Tosa
    Pages 155-162
  13. Peter Hammerl, Arnulf Hartl, Johannes Freund, Joseph Thalhamer
    Pages 163-173
  14. Neal F. Gordon, Duncan H. Whitney, Tom R. Londo, Tim K. Nadler
    Pages 175-193
  15. George K. Ehrlich, Wolfgang Berthold, Pascal Bailon
    Pages 195-208
  16. George K. Ehrlich, Pascal Bailon, Wolfgang Berthold
    Pages 209-220
  17. Back Matter
    Pages 221-230

About this book

Introduction

Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

Editors and affiliations

  • Pascal Bailon
    • 1
  • George K. Ehrlich
    • 1
  • Wen-Jian Fung
    • 1
  • Wolfgang Berthold
    • 1
  1. 1.Department of BiopharmaceuticalsHoffmann-La Roche Inc.Nutley

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-261-2
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-694-9
  • Online ISBN 978-1-60327-261-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site