Abstract
The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
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© 2006 Humana Press Inc., Totowa, NJ
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Altafaj, X., Joux, N., Ronjat, M., De Waard, M. (2006). Oocyte Expression With Injection of Purified T7 RNA Polymerase. In: Liu, X.J. (eds) Xenopus Protocols. Methods in Molecular Biology™, vol 322. Humana Press. https://doi.org/10.1007/978-1-59745-000-3_5
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DOI: https://doi.org/10.1007/978-1-59745-000-3_5
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