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Xenopus Protocols

Cell Biology and Signal Transduction

  • X. Johné Liu

Part of the Methods in Molecular Biology™ book series (MIMB, volume 322)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Steven L. Klein, Daniela S. Gerhard, Lukas Wagner, Paul Richardson, Lynn M. Schriml, Amy K. Sater et al.
    Pages 1-16
  3. Melissa A. Rasar, Stephen R. Hammes
    Pages 17-30
  4. X. Shawn Liu, X. Johné Liu
    Pages 31-41
  5. Jean-François L. Bodart, Nicholas S. Duesbery
    Pages 43-53
  6. Xavier Altafaj, Nathalie Joux, Michel Ronjat, Michel De Waard
    Pages 55-67
  7. Angela M. Prouty, Jun Wu, Da-Ting Lin, Patricia Camacho, James D. Lechleiter
    Pages 87-101
  8. Sheila L. Dargan, Angelo Demuro, Ian Parker
    Pages 103-119
  9. Danièle Roche, Geneviève Almouzni, Jean-Pierre Quivy
    Pages 139-147
  10. Kyong Hwa Moon, Xinliang Zhao, Yi-Tao Yu
    Pages 149-163
  11. David Stewart, Akihiro Tomita, Yun-Bo Shi, Jiemin Wong
    Pages 165-181
  12. Maria Piqué, José Manuel López, Raúl Méndez
    Pages 183-198
  13. Kathleen I. J. Sherman
    Pages 199-212
  14. John J. Heikkila, Angelo Kaldis, Rashid Abdulle
    Pages 213-222
  15. Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbé, Thierry Lorca
    Pages 223-234
  16. Reiner Peters
    Pages 235-258
  17. Bohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, Ueli Aebi
    Pages 273-288
  18. Catherine E. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris, Ulrike Laitko
    Pages 315-329
  19. Steven David Buckingham, Luanda Pym, David Barry Sattelle
    Pages 331-345
  20. Ricardo Miledi, Eleonora Palma, Fabrizio Eusebi
    Pages 347-355
  21. Andrea V. Gamarnik, Raul Andino
    Pages 367-378
  22. Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov, Tetsushi Iwasaki, Kazuyoshi Yonezawa, Yasuo Fukami
    Pages 395-411
  23. Richard F. Crane, Joan V. Ruderman
    Pages 435-443
  24. Keita Ohsumi, Tomomi M. Yamamoto, Mari Iwabuchi
    Pages 445-458
  25. Back Matter
    Pages 475-489

About this book

Introduction

Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses.

Keywords

Chaperone DNA Proteomics RNA Splicing Regulation Translation cell biology cellular processes physiology signal transduction transcription

Editors and affiliations

  • X. Johné Liu
    • 1
  1. 1.Ottawa Health Research InstituteOttawa HospitalOttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-59745-000-3
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-362-6
  • Online ISBN 978-1-59745-000-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site