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Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria

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Microbial Steroids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1645))

Abstract

The importance of the pathogenic mycobacteria has mainly focused the omic analyses on different aspects of their clinical significance. In contrast, those industrially relevant mycobacteria have received less attention, even though the steroids market sales in 2011, in example, were estimated in $8 billion.

The extra-cellular proteome, due to its relevance in the sterols processing and uptake; as well as the intra-cellular proteome, because of its role in steroids bioconversion, are the core of the present chapter. As a proof of concept, the obtaining methods for both sub-proteomes of Mycobacterium neoaurum NRRL B-3805, a relevant industrial strain involved in steroids production, have been developed. Thus, procedures and relevant key points of these proteomes analyses are fully described.

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Acknowledgments

This work was fully supported by a grant of the European Union program ERA-IB [MySterI (EIB.12.010)] through the APCIN call of the Spanish Ministry of Economy and Competitiveness (MINECO, Spain) (PCIN-2013-024-C02-01). The authors want to thank the European Union program ERA-IB; the Spanish Ministry of Economy and Competitiveness (MINECO, Spain); and the MySterI Consortium (INBIOTEC, Pharmins Ltd., University of York, SINTEF, Technische Universität Dortmund and Gadea Biopharma S.L.). We thank J. Merino, B. Martín, and A. Casenave for their excellent technical assistance and the degree students of the group A. Ibáñez and A. del Árbol.

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Authors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Avda. Real 1, 24006, León, Spain

    Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias & Alberto Sola-Landa

  2. Área de Microbiología, Departamento de Biología Molecular, Campus de Ponferrada, Universidad de León, Avda. Astorga, s/n, 24400, Ponferrada, Spain

    Carlos Barreiro

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  1. Carlos Barreiro
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  2. Alejandro Morales
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  3. Inés Vázquez-Iglesias
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  4. Alberto Sola-Landa
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Corresponding author

Correspondence to Carlos Barreiro .

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Editors and Affiliations

  1. Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

  2. Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Valladolid, Spain

    Ignacio Herráiz

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Barreiro, C., Morales, A., Vázquez-Iglesias, I., Sola-Landa, A. (2017). Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria. In: Barredo, JL., Herráiz, I. (eds) Microbial Steroids. Methods in Molecular Biology, vol 1645. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7183-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7183-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7182-4

  • Online ISBN: 978-1-4939-7183-1

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