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Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331

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Microbial Steroids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1645))

Abstract

The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc2155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.

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Author information

Authors and Affiliations

  1. Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

    Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García & Beatriz Galán

Authors
  1. Julia García-Fernández
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  2. Igor Martínez
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  3. Lorena Fernández-Cabezón
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  4. Carmen Felpeto-Santero
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  5. José-Luis García
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  6. Beatriz Galán
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Corresponding author

Correspondence to Beatriz Galán .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

  2. Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Valladolid, Spain

    Ignacio Herráiz

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García-Fernández, J., Martínez, I., Fernández-Cabezón, L., Felpeto-Santero, C., García, JL., Galán, B. (2017). Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331. In: Barredo, JL., Herráiz, I. (eds) Microbial Steroids. Methods in Molecular Biology, vol 1645. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7183-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7183-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7182-4

  • Online ISBN: 978-1-4939-7183-1

  • eBook Packages: Springer Protocols

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