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Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans

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Natural Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1643))

Abstract

Natural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis. Surprisingly, the binding molecule of Aa bacteria to the Aa_Mab was Aa chaperonin 60 or HSP60, a protein that is not only responsible for maintaining cellular proteins conformation, but also functions as a potent virulence factor prompting bone resorption in periodontitis and as a putative pathogenic factor in atherosclerosis.

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Acknowledgment

This study was funded by Sigrid Juselius Foundation and Finnish Foundation for Cardiovascular Research.

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Authors and Affiliations

  1. Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, 90014, Finland

    Chunguang Wang & Sohvi Hörkkö

  2. Medical Research Center and Nordlab Oulu, University Hospital and University of Oulu, Oulu, 90029, Finland

    Chunguang Wang & Sohvi Hörkkö

Authors
  1. Chunguang Wang
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  2. Sohvi Hörkkö
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Corresponding author

Correspondence to Chunguang Wang .

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Editors and Affiliations

  1. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Srinivas V. Kaveri

  2. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Jagadeesh Bayry

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Wang, C., Hörkkö, S. (2017). Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans . In: Kaveri, S., Bayry, J. (eds) Natural Antibodies. Methods in Molecular Biology, vol 1643. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7180-0_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7180-0_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7179-4

  • Online ISBN: 978-1-4939-7180-0

  • eBook Packages: Springer Protocols

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