Abstract
In vivo delivery of morpholino antisense oligonucleotides (MAO) directly into the uterine lumen of a peri-implantation period pregnant sheep is an effective technique for evaluation of gene products for conceptus development. The highly phagocytic conceptus is undergoing rapid morphological change, thereby the available MAO are readily consumed and delivered to developing cells. Here, we describe the method for preparation and surgical delivery of MAO—Endo-Porter complex to developing ovine conceptus on day 8 postmating. Also outlined are methods for posttreatment sample recovery on day 16 postmating.
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Acknowledgement
The authors would like to thank Fuller W. Bazer, M. Carey Satterfield, and Thomas E. Spencer, as well as former graduate students and postdoctoral fellows in the Bazer, Spencer and Satterfield laboratories for their participation in development of the in vivo MAO delivery method for use in pregnant sheep. This research was supported by the Agriculture and Food Research Initiative competitive grants 2011-6705-20028 and 2011–67015-20067 from the U.S. Department of Agriculture National Institute of Food and Agriculture, as well as support via National Institutes of Health grant HD052745.
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Wang, X., Dunlap, K.A. (2017). Delivery of Morpholino Antisense Oligonucleotides to a Developing Ovine Conceptus via Luminal Injection into a Ligated Uterine Horn. In: Moulton, H., Moulton, J. (eds) Morpholino Oligomers. Methods in Molecular Biology, vol 1565. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6817-6_20
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DOI: https://doi.org/10.1007/978-1-4939-6817-6_20
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Publisher Name: Humana Press, New York, NY
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