Morpholino Oligomers

Methods and Protocols

  • Hong M. Moulton
  • Jon D. Moulton

Part of the Methods in Molecular Biology book series (MIMB, volume 1565)

Table of contents

  1. Front Matter
    Pages i-x
  2. Garrick Chow, Paul A. Morcos, Hong M. Moulton
    Pages 31-38
  3. Alex Sutton Flynt, Mahesh Rao, James G. Patton
    Pages 59-78
  4. Ryan Thummel, M. Kathryn Iovine
    Pages 79-85
  5. Octavian Voiculescu, Claudio D. Stern
    Pages 105-114
  6. Seth M. Daly, Carolyn R. Sturge, David E. Greenberg
    Pages 115-122
  7. Jana Krtková, Alexander R. Paredez
    Pages 123-140
  8. Madzia P. Crossley, Torsten Krude
    Pages 151-160
  9. Rastislav Levicky, Ursula Koniges, Napoleon Tercero
    Pages 181-190
  10. Rika Maruyama, Yusuke Echigoya, Oana Caluseriu, Yoshitsugu Aoki, Shin’ichi Takeda, Toshifumi Yokota
    Pages 201-213
  11. Devi Krishna Priya Karunakaran, Rahul Kanadia
    Pages 215-227

About this book

Introduction

This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.

Keywords

modulating microRNA Morpholinos fin regeneration Electroporation Giardia lamblia

Editors and affiliations

  • Hong M. Moulton
    • 1
  • Jon D. Moulton
    • 2
  1. 1.Department of Biomedical Sciences College of Veterinary MedicineOregon State UniversityCorvallisUSA
  2. 2.Gene Tools, LLCPhilomathUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6817-6
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6815-2
  • Online ISBN 978-1-4939-6817-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book