Abstract
The microfluidic technology allows the production of network of submillimeter-size fluidic channels and reservoirs in a variety of material systems. The microfluidic-based polymerase chain reaction (PCR) allows automated multiplexing of multiple samples and multiple assays simultaneously within a network of microfluidic channels and chambers that are co-ordinated in controlled fashion by the valves. The individual PCR reaction is performed in nanoliter volume, which allows testing on samples with limited DNA and RNA. The microfluidics devices are used in various types of PCR such as digital PCR and single molecular emulsion PCR for genotyping, gene expression, and miRNA expression. In this chapter, the use of a microfluidics-based PCR for simultaneous screening of 14 known fusion transcripts in patients with leukemia is described.
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Acknowledgements
I would like to thank Dr. Rajyalakshmi Luthra, Dr. Rajesh Singh, Seema Hai, and molecular diagnostic laboratory at MD Anderson Cancer Center for providing figures and technical support.
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Chen, H. (2016). Microfluidics-Based PCR for Fusion Transcript Detection. In: Luthra, R., Singh, R., Patel, K. (eds) Clinical Applications of PCR. Methods in Molecular Biology, vol 1392. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3360-0_10
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DOI: https://doi.org/10.1007/978-1-4939-3360-0_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3360-0
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