Clinical Applications of PCR

  • Rajyalakshmi Luthra
  • Rajesh R. Singh
  • Keyur P. Patel

Part of the Methods in Molecular Biology book series (MIMB, volume 1392)

Table of contents

  1. Front Matter
    Pages i-x
  2. Zhuang Zuo, Kausar J. Jabbar
    Pages 17-25
  3. Meenakshi Mehrotra
    Pages 27-32
  4. Rashmi Kanagal-Shamanna
    Pages 33-42
  5. Rashmi Kanagal-Shamanna
    Pages 43-50
  6. Meenakshi Mehrotra, Keyur P. Patel
    Pages 63-69
  7. Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, Alberto Paniz Mondolfi
    Pages 113-124
  8. María Elizabeth Márquez, Juan Luis Concepción, Eglys González-Marcano, Alberto Paniz Mondolfi
    Pages 125-141
  9. Rashmi S. Goswami
    Pages 143-151
  10. Chi Young Ok, Rajesh R. Singh, Alaa A. Salim
    Pages 153-159
  11. Charanjeet Singh, Sinchita Roy-Chowdhuri
    Pages 161-176
  12. Khalida Wani, Kenneth D. Aldape
    Pages 177-186
  13. Back Matter
    Pages 187-191

About this book

Introduction

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

Keywords

Polymerase chain reaction Next Generation Sequencing DNA methylation Gene copy number aberrations Primer extension Genomics Transcriptomics Personalized medicine

Editors and affiliations

  • Rajyalakshmi Luthra
    • 1
  • Rajesh R. Singh
    • 2
  • Keyur P. Patel
    • 3
  1. 1.Anderson Cancer CenterThe University of Texas MDHoustonUSA
  2. 2.Anderson Cancer CenterThe University of Texas MDHoustonUSA
  3. 3.Anderson Cancer CenterThe University of Texas MDHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3360-0
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3358-7
  • Online ISBN 978-1-4939-3360-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book