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SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast

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SiRNA Delivery Methods

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1364))

Abstract

SiRNA therapeutics promise a future where any target in the transcriptome could be potentially addressed. However, the delivery of SiRNAs and targeting of particular cell types or organs are major challenges. A novel, efficient, and safe delivery system for promising the introduction of SiRNAs into particular cell types within living organisms is of great significance. Our previous studies have proved that recombinant protein (MSTN) and exogenous gene (EGFP) as vaccines, and furthermore functional CD40 shRNA expression can be delivered into dendritic cells (DCs) in mouse by oral administration of recombinant yeast (Saccharomyces cerevisiae). Here, we describe the details of the promising and innovative approach based on oral administration of recombinant yeast that allows in vivo-targeted delivery of functional SiRNA to murine intestinal DCs.

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Acknowledgments

The authors would like to thank the colleagues in Professor Zhang’s lab for their excellent technical assistance and helpful collaboration. We are grateful to financial support from China’s Ministry of Agriculture (948 Program 2013-Z27), China’s Ministry of Science and Technology (National Science and Technology Major Project 2014ZX0801009B and 973 Program 2011CBA01002) and National Natural Science Foundation of China (NSFC 31172186).

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    Authors and Affiliations

    1. College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China

      Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang & Zhiying Zhang

    2. Frontier Institute of Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, China

      Long Zhang

    3. Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, 030006, China

      Tingting Zhang

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    1. Kun Xu
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    2. Zhongtian Liu
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    3. Long Zhang
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    4. Tingting Zhang
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    5. Zhiying Zhang
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    Correspondence to Zhiying Zhang .

    Editor information

    Editors and Affiliations

    1. Beckman Research Institute of City of Hope, Duarte, California, USA

      Kato Shum

    2. Beckman Research Institute of City of Hope, Duarte, California, USA

      John Rossi

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    Xu, K., Liu, Z., Zhang, L., Zhang, T., Zhang, Z. (2016). SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast. In: Shum, K., Rossi, J. (eds) SiRNA Delivery Methods. Methods in Molecular Biology, vol 1364. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3112-5_14

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    • DOI: https://doi.org/10.1007/978-1-4939-3112-5_14

    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3111-8

    • Online ISBN: 978-1-4939-3112-5

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