Abstract
Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment’s notice.
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Gietz, R.D. (2014). Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_4
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DOI: https://doi.org/10.1007/978-1-4939-0799-1_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0798-4
Online ISBN: 978-1-4939-0799-1
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