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SiRNA Delivery Methods

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Kato Shum

    1. Beckman Research Institute of City of Hope, Duarte, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. John Rossi

    1. Beckman Research Institute of City of Hope, Duarte, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1364)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs

    • Alexander S. Hamil, Steven F. Dowdy
    Pages 1-9

  3. Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates

    • Stephanie E. Barrett, Erin N. Guidry
    Pages 11-25

  4. PepFect6 Mediated SiRNA Delivery into Organotypic Cultures

    • Suvarna Dash-Wagh, Ülo Langel, Mats Ulfendahl
    Pages 27-35

  5. Highly Efficient SiRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems

    • Lichen Yin, Nan Zheng, Jianjun Cheng
    Pages 37-47

  6. Disulfide-Bridged Cleavable PEGylation of Poly-l-Lysine for SiRNA Delivery

    • Min Tang, Haiqing Dong, Xiaojun Cai, Haiyan Zhu, Tianbin Ren, Yongyong Li
    Pages 49-61

  7. Preparation of a Cyclic RGD: Modified Liposomal SiRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells

    • Yu Sakurai, Tomoya Hada, Hideyoshi Harashima
    Pages 63-69

  8. A Multifunctional Envelope-Type Nano Device Containing a pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo

    • Yusuke Sato, Hideyoshi Harashima, Michinori Kohara
    Pages 71-78

  9. Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA

    • Kristen L. Kozielski, Jordan J. Green
    Pages 79-87

  10. Preparation of Polyion Complex Micelles Using Block Copolymers for SiRNA Delivery

    • Hyun Jin Kim, Meng Zheng, Kanjiro Miyata, Kazunori Kataoka
    Pages 89-103

  11. Delivery of Small Interfering RNAs to Cells via Exosomes

    • Jessica Wahlgren, Luisa Statello, Gabriel Skogberg, Esbjörn Telemo, Hadi Valadi
    Pages 105-125

  12. Dendrimer Nanovectors for SiRNA Delivery

    • Xiaoxuan Liu, Ling Peng
    Pages 127-142

  13. Chitosan Nanoparticles for SiRNA Delivery In Vitro

    • Héloïse Ragelle, Kevin Vanvarenberg, Gaëlle Vandermeulen, Véronique Préat
    Pages 143-150

  14. Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery

    • King Sun Siu, Yujuan Zhang, Xiufen Zheng, James Koropatnick, Wei-Ping Min
    Pages 151-163

  15. SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast

    • Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang, Zhiying Zhang
    Pages 165-181

  16. TLR9-Targeted SiRNA Delivery In Vivo

    • Dewan Md Sakib Hossain, Dayson Moreira, Qifang Zhang, Sergey Nechaev, Piotr Swiderski, Marcin Kortylewski
    Pages 183-196

  17. Aptamer-MiRNA Conjugates for Cancer Cell-Targeted Delivery

    • Carla L. Esposito, Silvia Catuogno, Vittorio de Franciscis
    Pages 197-208

  18. Method for Confirming Cytoplasmic Delivery of RNA Aptamers

    • David D. Dickey, Gregory S. Thomas, Justin P. Dassie, Paloma H. Giangrande
    Pages 209-217

  19. Hapten-Binding Bispecific Antibodies for the Targeted Delivery of SiRNA and SiRNA-Containing Nanoparticles

    • Irmgard S. Thorey, Michael Grote, Klaus Mayer, Ulrich Brinkmann
    Pages 219-234

  20. Stable Delivery of CCR5-Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector

    • Saki Shimizu, Swati Seth Yadav, Dong Sung An
    Pages 235-248
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Keywords

About this book

This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.

Editors and Affiliations

  • Beckman Research Institute of City of Hope, Duarte, USA

    Kato Shum, John Rossi

Bibliographic Information

  • Book Title: SiRNA Delivery Methods

  • Book Subtitle: Methods and Protocols

  • Editors: Kato Shum, John Rossi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3112-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3111-8Published: 16 October 2015

  • Softcover ISBN: 978-1-4939-4910-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3112-5Published: 15 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 313

  • Number of Illustrations: 29 b/w illustrations, 35 illustrations in colour

  • Topics: Human Genetics

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