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Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

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Baculovirus and Insect Cell Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1350))

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Abstract

By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the early-to-late (ETL) promoter of the virus vector. Because of its early appearance, GFP, when placed under the control of ETL promoter, will facilitate vector construction, virus isolation, and titer determination.

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Author information

Authors and Affiliations

  1. Fischell Department of Bioengineering, University of Maryland, 2226 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA

    William E. Bentley

  2. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA

    William E. Bentley

  3. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea

    Hyung Joon Cha

  4. Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617, Taiwan

    Hsuan-Chen Wu

  5. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA

    Hsuan-Chen Wu

Authors
  1. Hsuan-Chen Wu
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  2. Hyung Joon Cha
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  3. William E. Bentley
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Corresponding author

Correspondence to William E. Bentley .

Editor information

Editors and Affiliations

  1. Engineering, University of Iowa Dept. Chemical & Biochemical, Iowa City, Iowa, USA

    David W. Murhammer

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Wu, HC., Cha, H.J., Bentley, W.E. (2016). Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3043-2_22

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3042-5

  • Online ISBN: 978-1-4939-3043-2

  • eBook Packages: Springer Protocols

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