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Baculovirus and Insect Cell Expression Protocols

  • David W. Murhammer

Part of the Methods in Molecular Biology book series (MIMB, volume 1350)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

    1. Front Matter
      Pages 23-23
    2. Barbara J. Kelly, Linda A. King, Robert D. Possee
      Pages 25-50
    3. Robert D. Possee, Linda A. King
      Pages 51-71
    4. Linda A. King, Richard Hitchman, Robert D. Possee
      Pages 73-94
    5. James A. Fornwald, Quinn Lu, Frederick M. Boyce, Robert S. Ames
      Pages 95-116
  4. Insect Cell Culture

    1. Front Matter
      Pages 117-117
    2. Dwight E. Lynn, Robert L. Harrison
      Pages 119-142
    3. Leslie C. L. Chan, Steven Reid
      Pages 161-196
  5. Protein Production with Recombinant Baculoviruses

  6. Recombinant Protein Production with Transformed Insect Cells

    1. Front Matter
      Pages 327-327
  7. Baculovirus Development and Production for Use as Insecticides

    1. Front Matter
      Pages 381-381
    2. Holly J. R. Popham, Tyasning Nusawardani, Bryony C. Bonning
      Pages 383-392
    3. Nikolai van Beek, David C. Davis
      Pages 393-405
    4. Holly J. R. Popham, Mark R. Ellersieck, Huarong Li, Bryony C. Bonning
      Pages 407-444
  8. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

    1. Front Matter
      Pages 445-445
    2. Hsuan-Chen Wu, Hyung Joon Cha, William E. Bentley
      Pages 447-459
    3. Hsuan-Chen Wu, Yu-Chen Hu, William E. Bentley
      Pages 461-467
    4. Hsuan-Chen Wu, John C. March, William E. Bentley
      Pages 469-476
  9. Back Matter
    Pages 505-508

About this book

Introduction

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Keywords

BEVS RNAi baculovirus molecular biology biopesticides express genes generating envelop-modified baculovirus green fluorescent protein insect cell genome insect cell lines tubular reactors

Editors and affiliations

  • David W. Murhammer
    • 1
  1. 1.EngineeringUniversity of Iowa Dept. Chemical & BiochemicalIowa CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3043-2
  • Copyright Information Springer Science+Business Media, LLC 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3042-5
  • Online ISBN 978-1-4939-3043-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site