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Advanced Crystallographic Data Collection Protocols for Experimental Phasing

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Nucleic Acid Crystallography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1320))

Abstract

Experimental phasing by single- or multi-wavelength anomalous dispersion (SAD or MAD) has become the most popular method of de novo macromolecular structure determination. Continuous advances at third-generation synchrotron sources have enabled the deployment of rapid data collection protocols that are capable of recording SAD or MAD data sets. However, procedural simplifications driven by the pursuit of high throughput have led to a loss of sophistication in data collection strategies, adversely affecting measurement accuracy from the viewpoint of anomalous phasing. In this chapter, we detail optimized strategies for collecting high-quality data for experimental phasing, with particular emphasis on minimizing errors from radiation damage as well as from the instrument. This chapter also emphasizes data processing for “on-the-fly” decision-making during data collection, a critical process when data quality depends directly on information gathered while at the synchrotron.

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Author information

Authors and Affiliations

  1. Paul Scherrer Institute, Swiss Light Source, Villigen, 5232, Switzerland

    Aaron D. Finke, Ezequiel Panepucci, Meitian Wang & Vincent Oliéric

  2. Global Phasing Ltd, Cambridge, UK

    Clemens Vonrhein & Gérard Bricogne

Authors
  1. Aaron D. Finke
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  2. Ezequiel Panepucci
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  3. Clemens Vonrhein
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  4. Meitian Wang
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  5. Gérard Bricogne
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  6. Vincent Oliéric
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Corresponding author

Correspondence to Vincent Oliéric .

Editor information

Editors and Affiliations

  1. CNRS Inst de Biol Moléculaire et Cellulaire, Strasbourg, France

    Eric Ennifar

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Finke, A.D., Panepucci, E., Vonrhein, C., Wang, M., Bricogne, G., Oliéric, V. (2016). Advanced Crystallographic Data Collection Protocols for Experimental Phasing. In: Ennifar, E. (eds) Nucleic Acid Crystallography. Methods in Molecular Biology, vol 1320. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2763-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2763-0_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2762-3

  • Online ISBN: 978-1-4939-2763-0

  • eBook Packages: Springer Protocols

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