Nucleic Acid Crystallography

Methods and Protocols

  • Eric Ennifar
Part of the Methods in Molecular Biology book series (MIMB, volume 1320)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. RNA Preparation and Crystallogenesis

    1. Front Matter
      Pages 9-9
    2. Alla Peselis, Ang Gao, Alexander Serganov
      Pages 21-36
    3. Clément Dégut, Alexandre Monod, Franck Brachet, Thibaut Crépin, Carine Tisné
      Pages 37-57
    4. Eileen Sherman, Jennifer Archer, Jing-Dong Ye
      Pages 77-109
    5. Cyrielle Da Veiga, Joelle Mezher, Philippe Dumas, Eric Ennifar
      Pages 127-143
  4. Data Collection, Phasing and Refinement

    1. Front Matter
      Pages 145-145
    2. Kay Diederichs
      Pages 147-173
    3. Aaron D. Finke, Ezequiel Panepucci, Clemens Vonrhein, Meitian Wang, Gérard Bricogne, Vincent Oliéric
      Pages 175-191
    4. Chloe Zubieta, Max H. Nanao
      Pages 205-218
    5. Alexandre Urzhumtsev, Ludmila Urzhumtseva, Ulrich Baumann
      Pages 259-267

About this book

Introduction

This volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.
 

Keywords

DNA Macromolecule Production Nucleic Acid Crystallography Nucleic Acid Purification Nucleic Acid Synthesis RNA Crystallogenesis RNA Preparation Structure Determination X-Ray Crystallography

Editors and affiliations

  • Eric Ennifar
    • 1
  1. 1.CNRS Inst de Biol Moléculaire et CellulaireStrasbourgFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2763-0
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2762-3
  • Online ISBN 978-1-4939-2763-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book