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GC-MS-Based 13C Metabolic Flux Analysis

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Metabolic Flux Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1191))

Abstract

The in vivo analysis of metabolic fluxes has become a valuable method for the investigation of microorganisms. It turned out especially useful in industrial biotechnology for the prediction of beneficial genetic targets for rational strain optimization. Here, we describe in detail the procedure for the state-of-the-art approach of 13C metabolic flux analysis comprising steady-state cultivations in a mineral salt medium with 13C-labeled substrates, GC-MS measurement for labeling analysis, as well as metabolic modeling using the open-source software OpenFlux.

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References

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Authors and Affiliations

  1. Institute of Systems Biotechnology, Saarland University, Campus A 1 5, 66123, Saarbrücken, Germany

    Judith Becker & Christoph Wittmann

Authors
  1. Judith Becker
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  2. Christoph Wittmann
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Corresponding author

Correspondence to Christoph Wittmann .

Editor information

Editors and Affiliations

  1. Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management Centre, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Jens O. Krömer

  2. AIBN, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Lars K. Nielsen

  3. Biology Department, RWTH Aachen University, Aachen, Germany

    Lars M. Blank

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© 2014 Springer Science+Business Media New York

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Becker, J., Wittmann, C. (2014). GC-MS-Based 13C Metabolic Flux Analysis. In: Krömer, J., Nielsen, L., Blank, L. (eds) Metabolic Flux Analysis. Methods in Molecular Biology, vol 1191. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1170-7_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1170-7_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1169-1

  • Online ISBN: 978-1-4939-1170-7

  • eBook Packages: Springer Protocols

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