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Isolation of Synaptosomes from Archived Brain Tissues

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Current Laboratory Methods in Neuroscience Research

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Synapses in the central nervous system serve as communication points between neurons and are critical regulators of neurotransmission and synaptic plasticity, the latter refers to a process of experience dependent changes in synaptic connectivity, where neurons undergo extensive sculpting and rewiring. Research on understanding the changes at the level of the synapse holds great promise into understanding the biological basis of many neurodegenerative and neuropsychiatric disorders in which brain wiring goes awry. One such approach to understand the changes occurring at the synapse is by isolating synaptosomes. Here, we describe the isolation of synaptosomes from archived human brain tissue using subcellular fractionation, which when combined to high-throughput “omics”-based approaches could yield vital clues into understanding the underlying bases of neurodegeneration.

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Authors and Affiliations

  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198-5800, USA

    Gurudutt Pendyala, James L. Buescher & Howard S. Fox

Authors
  1. Gurudutt Pendyala
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  2. James L. Buescher
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  3. Howard S. Fox
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Corresponding author

Correspondence to Gurudutt Pendyala .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA

    Huangui Xiong  & Howard E. Gendelman  & 

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Pendyala, G., Buescher, J.L., Fox, H.S. (2014). Isolation of Synaptosomes from Archived Brain Tissues. In: Xiong, H., Gendelman, H.E. (eds) Current Laboratory Methods in Neuroscience Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8794-4_10

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  • DOI: https://doi.org/10.1007/978-1-4614-8794-4_10

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-8793-7

  • Online ISBN: 978-1-4614-8794-4

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