Abstract
To imitate in vivo environment of cells, microfluidics offer controllable fashions at micro-scale and enable regulate flow-related parameters precisely, leveraging the current state of 3D systems to 4D level through the inclusion of flow and shear stress. In particular, integrating silk fibroin as an adhering layer with microfluidic chips enables to form more comprehensive and biocompatible network between cells since silk fibroin holds outstanding mechanical and biological properties such as easy processability, biocompatibility, controllable biodegradation, and versatile functionalization. In this chapter, we describe design and fabrication of a microfluidic chip, with silk fibroin–covered microchannels for the formation of 3D structures, such as MCF-7 (human breast cancer) cell spheroids as a model system. All the steps performed here are characterized by surface-sensitive tools and standard tissue culture methods. Overall, this strategy can be easily integrated into various high-tech application areas such as drug delivery systems, regenerative medicine, and tissue engineering in near future.
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References
Sung H, Ferlay J, Siegel RL et al (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 71:209–249
Valente KP, Khetani S, Kolahchi AR et al (2017) Microfluidic technologies for anticancer drug studies. Drug Discov Today 22:1654–1670
Sontheimer-Phelps A, Hassell BA, Ingber DE (2019) Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer 19:65–81
Lv D, Hu Z, Lu L et al (2017) Three-dimensional cell culture: a powerful tool in tumor research and drug discovery. Oncol Lett 14:6999–7010
Kapałczyńska M, Kolenda T, Przybyła W et al (2018) 2D and 3D cell cultures – a comparison of different types of cancer cell cultures. Arch Med Sci 14:910–919
Li XJJ, Valadez AV, Zuo P et al (2012) Microfluidic 3D cell culture: potential application for tissue- based bioassays. Mol Cell Biochem 23:1–7
Zhang JZ, Nagrath S (2013) Microfluidics and cancer: are we there yet? Biomed Microdevices 15:595–609
Akceoglu GA, Saylan Y, Inci F (2021) A snapshot of microfluidics in point-of-care diagnostics: multifaceted integrity with materials and sensors. Adv Mater Technol 6:2100049
Ren K, Zhou J, Wu H (2013) Materials for microfluidic chip fabrication. Acc Chem Res 46:2396–2406
Toepke MW, Beebe DJ (2006) PDMS absorption of small molecules and consequences in microfluidic applications. Lab Chip 6:1484–1486
Nguyen TP, Nguyen QV, Nguyen VH et al (2019) Silk fibroin-based biomaterials for biomedical applications: a review. Polymers (Basel) 11:1–25
Bettinger CJ, Cyr KM, Matsumoto A et al (2007) Silk fibroin microfluidic devices. Adv Mater 19:2847–2850
Alheib O, da Silva LP, Youn YH et al (2021) 3D bioprinting: a step forward in creating engineered human tissues and organs. In: Pou J, Riveiro A, EDavim JP (eds) Additive manufacturing. Handbooks in advanced manufacturing. Elsevier, pp 599–633
Acknowledgments
Fatih Inci and Eylul Gulsen Yilmaz gratefully acknowledge the support from the Scientific and Technological Research Council of Turkey (TÜBİTAK) 2232 International Fellowship for Outstanding Researchers (Project No: 118C254). Fatih Inci thanks for the support from the Turkish Academy of Sciences Outstanding Young Scientists Award Program (TÜBA-GEBİP). This work was also supported by the Young Scientist Awards Program (BAGEP) award from the Science Academy.
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Yilmaz, E.G., Inci, F. (2024). 3D Breast Cancer Model on Silk Fibroin–Integrated Microfluidic Chips. In: Sumbalova Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 2764. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3674-9_16
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DOI: https://doi.org/10.1007/978-1-0716-3674-9_16
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