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SNIP1: a new activator of HSE signaling pathway

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Abstract

In the last 10 years, more and more attention has been focused on SNIP1 (Smad nuclear interacting protein 1), which functions as a transcriptional coactivator. We report here that through quantitative real-time PCR analysis in 18 different human tissues, SNIP1 was found to be expressed ubiquitously. When overexpressed in HeLa cells, SNIP1-EGFP fused protein exhibited a nuclear localization with a characteristic subnuclear distribution in speckles or formed larger discrete nuclear bodies in some cells. Reporter gene assay showed that overexpression of SNIP1 in HEK 293 cells or H1299 cells strongly activated the HSE signaling pathway. Moreover, SNIP1 could selectively regulate the transcription of HSP70A1A and HSP27. Taken together, our findings suggest that SNIP1 might also be a positive regulator of HSE signaling pathway.

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Abbreviations

SNIP1:

Smad nuclear interacting protein 1

HSP:

Heat shock proteins

HSE:

Heat shock elements

HSF:

Heat shock factor

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Acknowledgments

This work was supported by the National 973 program of China (2004CB518605), the National 863 project of China (2006AA020501), the National Key Sci-Tech Special Project of China (2008ZX10002-020), the Project of the Shanghai Municipal Science and Technology Commission (03dz14086), and the National Natural Science foundation of China (30024001, 30771188).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China

    Qiang Li, Jian An, Xianghua Liu, Mingjun Zhang, Yichen Ling, Chenji Wang, Jing Zhao & Long Yu

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  1. Qiang Li
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  2. Jian An
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  3. Xianghua Liu
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  8. Long Yu
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Correspondence to Long Yu.

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Li, Q., An, J., Liu, X. et al. SNIP1: a new activator of HSE signaling pathway. Mol Cell Biochem 362, 1–6 (2012). https://doi.org/10.1007/s11010-011-1120-y

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